BPC-157 Research Peptide — High-Purity Synthetic Pentadecapeptide for Laboratory Use

Product Overview

BPC-157 is a research-grade synthetic pentadecapeptide consisting of 15 amino acids. It is derived from a protective protein found in gastric juice and is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 5mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 5mg per vial, 10 vials per pack

• Form: Lyophilized (freeze-dried) powder

• Purity: >99% verified by third-party testing

• Molecular Formula: C₆₂H₉₈N₁₆O₂₂

• Molecular Mass: 1419.5

• CAS Number: 137525-51-0

• Sequence: Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (GEPPPGKPADDAGLV)

• Synonyms: BPC 157, PL 14736, PL-10

• Research Use Only: Not for human or animal consumption

Understanding BPC-157: The Research Context

BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide corresponding to a fragment of a naturally occurring gastric protein. It was initially identified through research into gastrointestinal mucosal protection and has since become one of the most extensively studied peptides in regenerative research.

Key Research Interests:

• Cytoprotective signaling and tissue protection mechanisms

• Angiogenesis and blood vessel formation

• Nitric oxide pathway modulation

• Tendon, ligament, and muscle healing models

• Gastrointestinal barrier integrity

• In vitro cell culture investigations

Researchers utilize BPC-157 in controlled laboratory settings to study its role in tissue protection, repair, and regeneration. The peptide is of particular interest to investigators focused on musculoskeletal repair, gastrointestinal health, and the fundamental mechanisms of tissue healing.

The Significance of BPC-157’s Origin:
BPC-157 was discovered through research into gastric juice proteins that protect the stomach lining from its own acidic environment. These proteins demonstrate remarkable stability, surviving exposure to extreme acidic conditions (pH 1.5-3.5). This stability is retained in the synthetic BPC-157 fragment, making it unusually resilient in laboratory settings compared to many other research peptides.

The “Body Protection” Concept:
The peptide’s name reflects its broad cytoprotective properties observed in research. Unlike many peptides that target specific receptors or pathways, BPC-157 appears to influence multiple aspects of tissue repair, including:

• Promoting blood vessel formation (angiogenesis)

• Modulating nitric oxide signaling

• Protecting cells from various forms of damage

• Supporting tissue organization during healing

Third-Party Testing and Quality Assurance

Every batch of BPC-157 undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third-party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high-performance liquid chromatography (HPLC)

• Batch-to-batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

• Endotoxin testing for cell culture applications

With a verified purity exceeding 99%, BPC-157 meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients.

Chemical and Physical Properties

Molecular Characteristics

BPC-157 is a synthetic 15-amino acid linear peptide with a specific sequence derived from a gastric protein fragment.

Detailed Molecular Information:

• Molecular Formula: C₆₂H₉₈N₁₆O₂₂

• Molecular Mass: 1419.5 daltons

• CAS Number: 137525-51-0

• Sequence: GEPPPGKPADDAGLV (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val)

• Number of Amino Acids: 15

• Isoelectric Point (pI): Approximately 4.5-5.0

Full Sequence Breakdown with Properties:

Structural Features:

• Linear pentadecapeptide (15 amino acids)

• Contains three consecutive prolines (positions 3-5) — creates structural rigidity

• Contains multiple negatively charged residues (glutamic acid, aspartic acid)

• Contains positively charged lysine

• Hydrophobic C-terminus (leucine, valine)

• No disulfide bonds or cyclic structures

The cluster of three consecutive prolines (Pro-Pro-Pro) is a notable structural feature. Proline is unique among amino acids because its side chain cycles back to bond with the amine group, creating a rigid ring structure. Three prolines in a row create a very stiff, structured region in the peptide, which may contribute to its stability and biological activity.

Physical Form and Storage

The product is supplied as a lyophilized (freeze-dried) powder within a sterile, sealed vial. The powder typically appears as a white to off-white substance.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long-term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze-thaw cycles once reconstituted

Stability Considerations:
BPC-157 is remarkably stable compared to many research peptides. The peptide was discovered in gastric fluid, which has a pH of 1.5-3.5 and contains proteolytic enzymes. BPC-157 retains the stability of its parent protein, resisting degradation under conditions that would rapidly break down typical peptides. This stability makes BPC-157 particularly suitable for extended research protocols.

Once reconstituted with an appropriate sterile solvent, the solution should be used according to laboratory protocols. Under refrigerated conditions, reconstituted BPC-157 maintains stability for extended periods compared to less stable peptides.

Intended Use and Regulatory Status

Research Use Only

BPC-157 is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating cytoprotective signaling pathways

• Angiogenesis research — studying blood vessel formation mechanisms

• Tissue repair studies — musculoskeletal and soft tissue healing models

• Gastrointestinal research — barrier integrity and mucosal protection

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into peptide structure and function

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

BPC-157 should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for BPC-157 5MG:
For researchers needing a 5mg/mL working solution, add 1mL of sterile solvent to the 5mg vial. For a 2.5mg/mL solution, add 2mL of solvent. For a 1mg/mL solution, add 5mL of solvent. For a 0.5mg/mL solution, add 10mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
BPC-157 is water-soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate-buffered saline (PBS) at neutral pH. Due to its exceptional stability, BPC-157 is compatible with a wide range of laboratory solvents.

Disposal Considerations

Dispose of BPC-157 and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

BPC-157 is used in various laboratory research contexts, spanning gastrointestinal biology, musculoskeletal repair, and angiogenesis research. Typical applications include:

Angiogenesis and Vascular Research:
BPC-157 has been studied for its effects on blood vessel formation. Researchers use BPC-157 to investigate:

• Vascular endothelial growth factor (VEGF) expression and activation

• Endothelial cell proliferation and migration

• Capillary formation and vascular density

• Blood flow restoration in compromised tissue models

Tendon, Ligament, and Musculoskeletal Studies:
BPC-157 has been extensively studied in musculoskeletal repair models. Research applications include:

• Tendon healing and collagen organization

• Ligament repair mechanisms

• Muscle regeneration and recovery

• Connective tissue healing dynamics

Gastrointestinal Barrier Research:
Given its origin in gastric proteins, BPC-157 is studied for gastrointestinal applications:

• Intestinal epithelial barrier integrity

• Mucosal protection mechanisms

• Inflammatory bowel disease models

• Leaky gut and permeability studies

Cytoprotective Signaling Studies:
BPC-157 influences multiple cellular protection pathways. Researchers use BPC-157 to study:

• Nitric oxide (NO) signaling modulation

• Pro-survival pathway activation

• Oxidative stress reduction mechanisms

• Cell death prevention pathways

Cell Culture Investigations:
BPC-157 is used in controlled cell culture systems to study:

• Fibroblast proliferation and migration

• Endothelial cell tube formation (angiogenesis assays)

• Epithelial cell barrier function

• Cellular responses to cytoprotective peptides

Nitric Oxide Pathway Research:
BPC-157 has been shown to modulate nitric oxide signaling. Research applications include:

• Endothelial nitric oxide synthase (eNOS) activation

• Inducible nitric oxide synthase (iNOS) regulation

• NO-mediated vasodilation studies

• Nitric oxide donor and antagonist interactions

In Vitro Wound Healing Models:
Researchers use BPC-157 to study:

• Scratch wound closure assays

• Cell migration and proliferation kinetics

• Growth factor expression following injury

• Extracellular matrix remodeling

Gastrointestinal Cell Studies:
BPC-157 is used in gastric and intestinal cell cultures to investigate:

• Mucosal cell protection against damaging agents

• Barrier function enhancement

• Inflammatory cytokine modulation

• Tight junction protein expression

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to BPC-157.

What Makes This Product Different

Exceptional Stability

Unlike most research peptides that degrade rapidly in biological environments, BPC-157 retains the remarkable stability of its gastric protein origin. It remains stable at pH 1.5-3.5 and resists proteolytic degradation, making it uniquely suitable for extended research protocols.

Broad Cytoprotective Profile

BPC-157 influences multiple tissue protection and repair pathways simultaneously, including angiogenesis, nitric oxide signaling, and cell survival pathways. This broad profile distinguishes BPC-157 from peptides with narrow, receptor-specific actions.

Extensive Research History

BPC-157 has been the subject of research for decades, with a substantial body of published literature supporting its characterization. This extensive research history provides a strong foundation for new investigations.

Optimal 5MG Research Quantity

The 5mg vial size provides researchers with sufficient quantity for multiple experiments, including cell culture studies, angiogenesis assays, and dose-response investigations.

Rigorous Quality Control

Unlike research chemicals sold without verification, BPC-157 comes with documented third-party testing for every batch. This commitment to quality assurance gives researchers confidence in the consistency and reliability of their experimental results.

Complete Documentation

Each product listing includes full chemical specifications, including molecular formula, molecular mass, CAS number, and amino acid sequence. This transparency allows researchers to verify the exact compound they are working with.

Appropriate Packaging for Research Use

The sterile, sealed vial packaging protects the lyophilized powder from environmental contaminants. The 5mg concentration is optimized for laboratory research applications.

Batch Traceability

Each package of BPC-157 includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of BPC-157?

Each batch is tested to verify purity exceeding 99%. Individual certificates of analysis are available upon request.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Avoid repeated freeze-thaw cycles once reconstituted.

What solvent should be used for reconstitution?

BPC-157 is water-soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as PBS at neutral pH.

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22-micron filter may be appropriate.

What is the significance of the CAS number 137525-51-0?

The CAS number is a unique identifier for BPC-157, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications.

What is the amino acid sequence of BPC-157?

The sequence is GEPPPGKPADDAGLV (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val).

What does BPC stand for?

BPC stands for “Body Protection Compound,” reflecting the peptide’s cytoprotective properties observed in research.

Why is BPC-157 unusually stable?

BPC-157 is derived from a gastric protein that naturally survives exposure to stomach acid (pH 1.5-3.5) and digestive enzymes. The synthetic peptide retains this remarkable stability.

What research applications is BPC-157 used for?

BPC-157 is studied in angiogenesis research, tendon and ligament repair models, gastrointestinal barrier studies, cytoprotective signaling investigations, and cell culture wound healing assays.

Can BPC-157 be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. BPC-157 is frequently studied in combination with GHK-Cu and TB-500 in multi-peptide formulations such as GLOW and KLOW. Standard laboratory practice includes testing interactions in control experiments.

Final Notes for Laboratory Researchers

BPC-157 represents a high-purity research tool for qualified professionals conducting legitimate scientific investigations into cytoprotective signaling, angiogenesis, tissue repair mechanisms, gastrointestinal barrier function, and related regenerative fields. With documented third-party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 5mg vial size is optimized for research applications requiring precise handling for cell culture studies, angiogenesis assays, and dose-response investigations. As a peptide derived from a remarkably stable gastric protein, BPC-157 offers unique research opportunities in tissue protection and regeneration.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.