BAC Water 30mL — Sterile Bacteriostatic Water for Research Peptide Reconstitution

Product Overview

BAC Water (Bacteriostatic Water) is a sterile, non-pyrogenic water solution containing 0.9% benzyl alcohol as a bacteriostatic preservative. It is supplied in multi-dose vials designed for repeated withdrawals in laboratory research settings. Each package contains ten vials, with each vial holding 30mL of sterile bacteriostatic water. This product is manufactured exclusively for research use and in vitro laboratory applications .

Key Specifications at a Glance:

• Quantity: 30mL per vial, 10 vials per pack

• Form: Ready-to-use sterile liquid

• Preservative: 0.9% Benzyl Alcohol (9mg/mL)

• Sterilization: 0.22µm filtration, aseptically filled

• pH Range: 4.5–7.0

• In-Use Period: Up to 28 days after first puncture (with aseptic handling)

• Storage: 20–25°C (Controlled Room Temperature)

• Research Use Only: Not for human or animal injection

Understanding BAC Water: The Research Context

BAC Water is a specialized sterile water solution designed for multiple withdrawals in laboratory research settings. Unlike standard sterile water (which is intended for single use), BAC Water contains 0.9% benzyl alcohol as a bacteriostatic preservative that inhibits bacterial growth, allowing the vial to be used repeatedly over a period of up to 28 days when proper aseptic technique is followed .

Key Research Applications:

• Peptide reconstitution and dilution

• Protein and antibody preparation

• Working solution preparation from lyophilized materials

• Laboratory reagent dissolution

• Cell culture media preparation

Researchers utilize BAC Water in controlled laboratory settings to reconstitute lyophilized (freeze-dried) research compounds, including peptides, proteins, and antibodies. The bacteriostatic preservative maintains sterility during repeated use, making it particularly valuable for research protocols requiring multiple samplings from the same vial .

The Role of Benzyl Alcohol:
The 0.9% benzyl alcohol concentration serves as a bacteriostatic agent, preventing the growth of contaminating bacteria in the solution after the vial has been opened. This allows for repeated withdrawals without compromising sterility, provided aseptic technique is maintained. The concentration of 0.9% (9 mg/mL) is well-established in pharmaceutical and research applications .

BAC Water vs. Sterile Water: Understanding the Difference

Researchers often ask about the distinction between BAC Water and standard sterile water. Understanding this difference is important for proper experimental design.

Choosing the Right Water for Your Research:

• BAC Water — Ideal for peptide reconstitution when multiple samplings from the same vial are required over days or weeks. The bacteriostatic preservative maintains sterility for up to 28 days .

• Sterile Water (Preservative-Free) — Appropriate for single-use applications where the entire contents are used immediately and no preservative is desired.

Quality Assurance and Manufacturing Standards

Every batch of BAC Water is manufactured under strict quality control standards to ensure sterility, consistency, and reliability for research applications.

Manufacturing Process:

• Sterilization: Purified using a 0.22 µm filtration process

• Filling: Aseptically filled into sterile vials

• Quality Standards: Manufactured in GMP-certified facilities under ISO standards

• Testing: Each batch undergoes sterility and endotoxin testing

Quality Specifications:

Physical Properties and Storage

Chemical Composition

Storage Conditions

Recommended Storage:

• Store at 20–25°C (68–77°F) — Controlled Room Temperature

• Protect from light exposure

• Do not freeze

• Keep vial sealed when not in use

After Opening:

• Use within 28 days of first puncture

• Refrigeration after opening is recommended by some manufacturers

• Always use aseptic technique when withdrawing solution

• Discard immediately if solution appears discolored or contains particles

Intended Use and Regulatory Status

Research Use Only

BAC Water is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• Peptide reconstitution — dissolving lyophilized research peptides

• Protein preparation — diluting proteins for laboratory assays

• Working solution preparation — creating stock solutions from lyophilized materials

• Cell culture applications — as a solvent for certain research reagents

• Analytical research — chemical analysis and characterization studies

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities .

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

BAC Water should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

Aseptic Technique Protocol

To maintain sterility and maximize the 28-day in-use period:

1. Before first use: Inspect vial for damage or turbidity. Discard if solution is discolored or contains particles .

2. Clean the stopper: Wipe the rubber stopper with a sterile alcohol swab before each puncture.

3. Use sterile needles/syringes: Always use sterile, single-use needles and syringes.

4. Minimize punctures: Avoid unnecessary punctures to reduce contamination risk.

5. Proper withdrawal: Insert needle through the center of the stopper. Do not touch the needle to any non-sterile surface.

6. Label with date: Write the date of first puncture on the vial label.

7. Discard after 28 days: Do not use beyond 28 days after first puncture, even if solution remains .

Peptide Reconstitution Protocol

When reconstituting lyophilized research peptides with BAC Water:

1. Allow the sealed BAC Water vial to reach room temperature.

2. Clean the rubber stopper with an alcohol swab.

3. Using a sterile syringe, withdraw the calculated volume of BAC Water needed for your peptide concentration.

4. Inject the BAC Water slowly down the inner wall of the peptide vial to avoid foaming.

5. Gently swirl the vial — do not shake vigorously.

6. Allow complete dissolution before use.

Important Considerations:

• Consult the peptide manufacturer’s datasheet for recommended reconstitution volume

• Some peptides may be incompatible with benzyl alcohol; verify compatibility before use

• For peptides sensitive to benzyl alcohol, preservative-free sterile water may be more appropriate

Disposal Considerations

Dispose of BAC Water and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

BAC Water is used in various laboratory research contexts, primarily for reconstituting lyophilized research compounds. Typical applications include:

Peptide Reconstitution:
The primary research application for BAC Water is dissolving lyophilized (freeze-dried) research peptides. The 0.9% benzyl alcohol concentration maintains sterility during repeated use, making it ideal for research protocols requiring multiple samplings over days or weeks .

Protein and Antibody Preparation:
BAC Water is used to prepare working solutions from lyophilized proteins and antibodies in laboratory research settings. The preservative maintains sterility during extended use protocols .

Working Solution Preparation:
Researchers use BAC Water to create stock solutions from lyophilized materials for various laboratory applications, including:

• Assay development

• Cell culture supplementation

• Analytical standards preparation

Laboratory Reagent Dissolution:
BAC Water serves as an aqueous vehicle for dissolving research reagents that require dilution or must be dissolved prior to use in laboratory experiments .

Cell Culture Media Preparation:
In some research applications, BAC Water may be used as a component in preparing cell culture media or buffers, though compatibility should always be verified.

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to their research applications.

What Makes This Product Different

Bacteriostatic Preservation for Multi-Use

Unlike standard sterile water that must be discarded after a single use, BAC Water contains 0.9% benzyl alcohol that inhibits bacterial growth for up to 28 days after first puncture. This allows researchers to use a single vial multiple times, reducing waste and improving laboratory efficiency .

Optimal pH Range for Peptide Compatibility

With a pH range of 4.5–7.0, BAC Water is compatible with a wide range of research peptides and proteins . This broad pH compatibility makes it suitable for reconstituting various research compounds.

GMP-Certified Manufacturing

BAC Water is manufactured in GMP-certified facilities under ISO standards, ensuring consistent quality and sterility for research applications .

Multi-Dose Vial Design

The 30mL multi-dose vial is specifically designed for repeated withdrawals in laboratory settings. The bromobutyl rubber closure provides a secure seal that maintains sterility when proper aseptic technique is used .

0.22µm Sterile Filtration

Each batch undergoes 0.22µm filtration and is aseptically filled, ensuring sterility and non-pyrogenicity for sensitive research applications .

28-Day In-Use Period

When proper aseptic technique is followed, each vial maintains sterility for up to 28 days after first puncture, providing flexibility for extended research protocols .

Frequently Asked Questions for Researchers

What is the benzyl alcohol concentration in BAC Water?

BAC Water contains 0.9% benzyl alcohol (9 mg/mL) as a bacteriostatic preservative .

How long can I use a vial after first puncture?

With proper aseptic handling, BAC Water can be used for up to 28 days after the first puncture. After this period, the vial should be discarded .

How should BAC Water be stored?

Store at 20–25°C (Controlled Room Temperature), protect from light, and do not freeze. After opening, refrigeration is recommended by some manufacturers .

What is the pH range of BAC Water?

The pH typically falls between 4.5 and 7.0 .

Is BAC Water suitable for all peptide reconstitution?

BAC Water is widely used for peptide reconstitution in research workflows. However, researchers should verify compatibility with their specific peptide, as some peptides may be sensitive to benzyl alcohol .

What is the difference between BAC Water and sterile water?

BAC Water contains 0.9% benzyl alcohol as a preservative and is supplied in multi-dose vials for repeated use (up to 28 days). Sterile water contains no preservative and is supplied in single-dose vials that must be discarded after one use .

Can BAC Water be used for cell culture applications?

Researchers should determine compatibility based on their specific experimental protocol. The benzyl alcohol preservative may affect certain cell culture systems.

What should I do if the solution appears discolored or contains particles?

Discard the vial immediately if the solution appears discolored or contains particles. Do not use .

Is BAC Water sterile?

Yes, BAC Water is sterile and non-pyrogenic, manufactured using 0.22µm filtration and aseptic filling processes .

What is the shelf life of unopened BAC Water?

Unopened BAC Water has a shelf life of approximately 24 months when stored properly at controlled room temperature .

Final Notes for Laboratory Researchers

BAC Water (30mL) represents a high-quality, sterile bacteriostatic water solution for qualified professionals conducting legitimate scientific investigations requiring peptide reconstitution, protein preparation, and other laboratory applications. With documented quality assurance, complete specifications, and appropriate packaging for research use, this product meets the standards expected by serious researchers.

The 30mL multi-dose vial size is optimized for research applications requiring repeated withdrawals over an extended period. The 0.9% benzyl alcohol preservative maintains sterility for up to 28 days when proper aseptic technique is followed, providing flexibility and efficiency for laboratory research protocols.

For research use only. Not for human or animal injection. Handle only in appropriate laboratory facilities with proper safety equipment and training.