AOD9604 Research Peptide — High-Purity hGH Fragment for Laboratory Use

Product Overview

AOD9604 is a research-grade synthetic peptide derived from the C-terminal fragment of human growth hormone (hGH). It corresponds to amino acids 177-191 of hGH with an additional tyrosine residue at the N-terminus, making it a 16-amino acid peptide also known as Tyr-hGH Frag 176-191 . It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 10mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 10mg per vial, 10 vials per pack

• Form: Lyophilized (freeze-dried) powder

• Purity: >98% verified by third-party testing

• Molecular Formula: C₇₈H₁₂₃N₂₃O₂₃S₂

• Molecular Mass: 1815.1

• CAS Number: 221231-10-3

• Sequence: Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly (cyclic 7→14 disulfide)

• Synonyms: hGH frag 176-191, AOD-9604, Tyr-somatostatin (177-191)

• Research Use Only: Not for human or animal consumption

Understanding AOD9604: The Research Context

AOD9604 is a synthetic peptide fragment derived from the C-terminus of human growth hormone. The name AOD stands for “Anti-Obesity Drug,” reflecting its initial research focus on metabolic regulation . Unlike full-length hGH, which affects multiple pathways including insulin-like growth factor (IGF-1) signaling, AOD9604 was developed to selectively target lipid metabolism pathways without significantly impacting insulin or IGF-1 levels .

Key Research Interests:

• Lipid metabolism and lipolysis regulation

• Adipose tissue biology and fat oxidation

• Cartilage repair and osteoarthritis models

• Bone metabolism and osteoporosis research

• In vitro cell culture investigations

Researchers utilize AOD9604 in controlled laboratory settings to study its selective effects on fat metabolism and tissue regeneration. The peptide is of particular interest to investigators focused on metabolic disorders, orthopedic research, and the fundamental mechanisms of growth hormone fragment biology.

The Significance of AOD9604’s Selectivity:
Full-length human growth hormone has well-documented effects on fat metabolism but also influences blood sugar regulation and tissue growth. AOD9604 was engineered to isolate the lipolytic (fat-burning) properties of hGH while avoiding the diabetogenic side effects associated with elevated IGF-1 and insulin resistance . This selectivity makes AOD9604 a valuable research tool for studying fat metabolism independently of other GH-mediated pathways.

Mechanism of Action:
AOD9604 is believed to work through a mechanism independent of the classical hGH receptor. It does not require the hGH receptor to activate fat breakdown, having its own distinct mechanism of action . The peptide appears to:

• Stimulate lipolysis (breakdown of stored fat)

• Inhibit lipogenesis (formation of new fat deposits)

• Activate fat oxidation pathways in adipose tissue

Third-Party Testing and Quality Assurance

Every batch of AOD9604 undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third-party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high-performance liquid chromatography (HPLC) — ≥98% purity

• Batch-to-batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

• Disulfide bond verification for the cyclic structure (7→14)

With a verified purity exceeding 98%, AOD9604 meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients.

Chemical and Physical Properties

Molecular Characteristics

AOD9604 is a synthetic 16-amino acid peptide with a cyclic structure stabilized by a disulfide bond between cysteine residues at positions 7 and 14 .

Detailed Molecular Information:

• Molecular Formula: C₇₈H₁₂₃N₂₃O₂₃S₂

• Molecular Mass: 1815.1 daltons

• CAS Number: 221231-10-3

• PubChem CID: 91810664

• Sequence: Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly (cyclic 7→14 disulfide)

• Purity: >98% (HPLC)

Full Sequence Breakdown with Properties:

Structural Features:

• 16-amino acid linear peptide with cyclic disulfide bridge

• Disulfide bond between cysteine residues at positions 7 and 14

• N-terminal tyrosine residue (not present in native hGH fragment)

• Contains both positively and negatively charged residues

• Hydrophobic residues in the mid-region

The cyclic structure created by the disulfide bond (Cys7–Cys14) is a notable feature that contributes to the peptide’s stability and biological activity . This cyclic conformation may protect the peptide from rapid enzymatic degradation compared to fully linear fragments.

Physical Form and Storage

The product is supplied as a lyophilized (freeze-dried) powder within a sterile, sealed vial. The powder typically appears as a white to off-white substance.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long-term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze-thaw cycles once reconstituted

Solubility Information:
AOD9604 is slightly soluble in DMSO and water . Researchers should test solubility in their chosen buffer system before proceeding with experiments.

Once reconstituted with an appropriate sterile solvent, the solution should be used promptly according to laboratory protocols.

Intended Use and Regulatory Status

Research Use Only

AOD9604 is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating lipolysis and metabolic pathways

• Metabolic research — studying fat oxidation and adipose tissue biology

• Cartilage and bone research — osteoarthritis and osteoporosis models

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into growth hormone fragment biology

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

WADA Status: AOD9604 is listed on the World Anti-Doping Agency (WADA) Prohibited List as a peptide hormone. It is banned in competitive sports .

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational and research purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

AOD9604 should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for AOD9604 10MG:
For researchers needing a 10mg/mL working solution, add 1mL of sterile solvent to the 10mg vial. For a 5mg/mL solution, add 2mL of solvent. For a 2mg/mL solution, add 5mL of solvent. For a 1mg/mL solution, add 10mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
AOD9604 has limited water solubility. Researchers should test solubility in their chosen buffer system. Common options include DMSO for stock solutions, followed by dilution into aqueous buffers. Phosphate-buffered saline (PBS) at neutral pH may be suitable for some applications .

Disposal Considerations

Dispose of AOD9604 and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

AOD9604 is used in various laboratory research contexts, spanning metabolic science, orthopedics, and regenerative biology. Typical applications include:

Lipid Metabolism and Adipose Tissue Research:
The primary research interest in AOD9604 revolves around its selective effects on fat metabolism. Researchers use AOD9604 to study:

• Lipolysis (fat breakdown) pathways in adipocyte cultures

• Lipogenesis inhibition mechanisms

• Fatty acid oxidation and energy expenditure

• Adipose tissue gene expression changes

In research models, AOD9604 has been shown to decrease body weight and adipose tissue mass . The peptide appears to trigger fat release from obese fat cells predominantly more than from lean cells .

Cartilage Repair and Osteoarthritis Research:
AOD9604 has been investigated for its potential in cartilage regeneration. Research applications include:

• Collagen synthesis and extracellular matrix remodeling

• Chondrocyte proliferation and differentiation

• Cartilage repair in osteoarthritis models

• Joint dysfunction and pain studies

In a rabbit model of collagenase-induced knee osteoarthritis, AOD9604 enhanced cartilage regeneration . Research in rats has shown that AOD9604 injections directly into arthritic joints can help improve pain and lessen disability .

Bone Metabolism and Osteoporosis Research:
AOD9604 has been studied for its skeletal effects. In an aged rat model of postmenopausal osteoporosis, AOD9604 treatment resulted in significantly higher femoral and vertebral bone mineral density compared to untreated controls. The peptide preserved cortical bone strength and stiffness .

Research applications in bone science include:

• Osteoblast and osteoclast activity studies

• Bone mineral density investigations

• Cortical bone metabolism

• Osteoporosis prevention models

Regenerative Science and Wound Healing:
Emerging research suggests AOD9604 may influence tissue repair processes. Research applications include:

• Cellular repair and regeneration mechanisms

• Progenitor cell proliferation and differentiation

• Wound healing and skin cell regeneration

• Extracellular matrix remodeling

Metabolite Identification and Analytical Studies:
AOD9604 has been characterized for its metabolic stability. Research has identified six potential metabolites after incubation in serum and urine, with one stable metabolite (consisting of amino acids CRSVEGSCG) being significantly more stable than the parent compound . These findings are relevant for:

• Peptide stability studies

• Analytical method development

• Detection window investigations

Cell Culture Investigations:
Researchers use AOD9604 in controlled cell culture systems to study:

• Adipocyte differentiation and function

• Chondrocyte metabolism and matrix production

• Osteoblast activity and mineralization

• Cellular responses to growth hormone fragments

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to growth hormone-derived peptides.

What Makes This Product Different

Selective Lipolytic Activity

Unlike full-length human growth hormone, AOD9604 was specifically designed to isolate fat-burning properties while avoiding effects on blood sugar and tissue growth. This selectivity makes it a valuable research tool for studying lipid metabolism independently of other GH-mediated pathways .

Distinct Mechanism of Action

AOD9604 does not require the classical hGH receptor to activate fat breakdown. It has its own distinct mechanism of action, operating independently of hGH receptor signaling . This unique pathway distinguishes AOD9604 from other growth hormone-derived research tools.

Cyclic Disulfide Structure

The disulfide bridge between cysteine residues at positions 7 and 14 creates a cyclic conformation that contributes to the peptide’s stability and biological activity . This structural feature is important for maintaining the peptide’s active conformation.

Multi-System Research Potential

AOD9604 has been investigated across multiple research domains, including:

• Metabolic research (lipolysis, lipogenesis, fat oxidation)

• Orthopedic research (cartilage repair, osteoarthritis)

• Bone research (osteoporosis, cortical bone metabolism)

• Regenerative science (wound healing, tissue repair)

This versatility makes AOD9604 a valuable tool for diverse research applications .

Well-Characterized Research History

AOD9604 has been the subject of extensive research, including studies in rodents, pigs, dogs, and humans. It has been investigated for its effects on body fat reduction, cartilage repair, and bone metabolism .

Rigorous Quality Control

Unlike research chemicals sold without verification, AOD9604 comes with documented third-party testing for every batch. This commitment to quality assurance gives researchers confidence in the consistency and reliability of their experimental results.

Complete Documentation

Each product listing includes full chemical specifications, including molecular formula, molecular mass, CAS number, and amino acid sequence. This transparency allows researchers to verify the exact compound they are working with.

Batch Traceability

Each package of AOD9604 includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of AOD9604?

Each batch is tested to verify purity exceeding 98% by HPLC. Individual certificates of analysis are available upon request.

What is the significance of the CAS number 221231-10-3?

The CAS number is a unique identifier for AOD9604, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications.

What is the amino acid sequence of AOD9604?

The sequence is Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly, with a disulfide bridge between the two cysteine residues (positions 7 and 14).

How does AOD9604 differ from full-length human growth hormone?

AOD9604 is a 16-amino acid fragment of hGH that selectively targets lipid metabolism pathways without significantly affecting insulin, IGF-1 levels, or tissue growth .

What is the disulfide bond in AOD9604?

AOD9604 contains a disulfide bridge between cysteine residues at positions 7 and 14, creating a cyclic structure that contributes to the peptide’s stability and biological activity .

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Avoid repeated freeze-thaw cycles once reconstituted .

What solvent should be used for reconstitution?

AOD9604 has limited water solubility. Researchers should test solubility in their chosen buffer system. DMSO is suitable for stock solutions, followed by dilution into aqueous buffers .

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22-micron filter may be appropriate.

What research applications is AOD9604 used for?

AOD9604 is studied in lipid metabolism research, adipose tissue biology, cartilage repair models, osteoarthritis investigations, bone metabolism studies (including osteoporosis), and regenerative science applications .

Can AOD9604 be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. Some research has explored AOD9604 in combination with BPC-157 for cartilage repair applications . Standard laboratory practice includes testing interactions in control experiments before full-scale implementation.

Final Notes for Laboratory Researchers

AOD9604 represents a high-purity research tool for qualified professionals conducting legitimate scientific investigations into lipid metabolism, adipose tissue biology, cartilage repair, bone metabolism, and related fields. With documented third-party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 10mg vial size is optimized for research applications requiring precise handling for cell culture studies, metabolic assays, and dose-response investigations. As a selectively acting fragment of human growth hormone, AOD9604 offers unique research opportunities for studying fat metabolism and tissue regeneration pathways independently of full-length GH effects.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.