SEMAX Research Peptide — Synthetic Nootropic ACTH(4-10) Analog for Laboratory Use

Product Overview

SEMAX is a research-grade synthetic heptapeptide derived from the N-terminal fragment (4-10) of adrenocorticotropic hormone (ACTH). It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 10mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation .

Key Specifications at a Glance:

• Quantity: 10mg per vial, 10 vials per pack

• Form: Lyophilized (freeze-dried) powder

• Purity: >98% verified by third-party testing

• Molecular Formula: C₃₇H₅₁N₉O₁₀S

• Molecular Mass: 813.92

• CAS Number: 80714-61-0

• Sequence: Met-Glu-His-Phe-Pro-Gly-Pro (MEHFPGP)

• Research Use Only: Not for human or animal consumption

Understanding SEMAX: The Research Context

SEMAX is a synthetic heptapeptide that was first developed by researchers in the 1980s. It is an analog of the ACTH(4-10) peptide fragment, which itself is derived from adrenocorticotropic hormone (ACTH), a hormone that plays a key role in cortisol production in the body .

Key Research Interests:

• Nootropic (cognitive enhancement) properties

• Neuroprotective mechanisms

• Brain-derived neurotrophic factor (BDNF) modulation

• Neurotransmitter system interactions

• In vitro cell culture investigations

Researchers utilize SEMAX in controlled laboratory settings to better understand the molecular mechanisms underlying its nootropic and neuroprotective properties. The peptide is of particular interest to investigators focused on neuroscience, neuropharmacology, and cellular signaling pathways .

The Significance of the ACTH(4-10) Backbone:
The ACTH(4-10) fragment (Met-Glu-His-Phe-Arg-Trp-Gly) is known to influence learning and memory processes. SEMAX is a stabilized analog of this fragment, with the C-terminus modified by the addition of the biogenic tripeptide Pro-Gly-Pro, which affords greater metabolic stability compared to the native sequence. This structural modification allows researchers to study the effects of a more stable ACTH analog in laboratory settings .

Third-Party Testing and Quality Assurance

Every batch of SEMAX undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third-party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release .

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high-performance liquid chromatography (HPLC)

• Batch-to-batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity exceeding 98%, SEMAX meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients.

Chemical and Physical Properties

Molecular Characteristics

SEMAX is a synthetic heptapeptide with the amino acid sequence Methionine-Glutamic Acid-Histidine-Phenylalanine-Proline-Glycine-Proline (MEHFPGP) .

Detailed Molecular Information:

• Molecular Formula: C₃₇H₅₁N₉O₁₀S

• Molecular Mass: 813.92 daltons

• CAS Number: 80714-61-0

• Synonyms: ACTH (4-7), Pro-Gly-Pro-; Met-Glu-His-Phe-Pro-Gly-Pro

The presence of sulfur in the molecular formula (from the methionine residue) indicates that SEMAX contains a sulfur-containing amino acid. This characteristic contributes to the compound’s specific three-dimensional structure and stability profile.

Physical Form and Storage

The product is supplied as a lyophilized (freeze-dried) powder within a sterile, sealed vial. Lyophilization preserves the chemical integrity of the peptide by removing water content while maintaining the compound’s native structure. This form allows for extended stability when stored properly .

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long-term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze-thaw cycles once reconstituted

Stability Considerations:
Peptides containing methionine (a sulfur-containing amino acid) can be more susceptible to oxidation than those without. For this reason, minimizing air exposure and avoiding repeated opening of stock vials is particularly important for SEMAX. Consider aliquoting reconstituted material under inert gas if extended storage is required.

Once reconstituted with an appropriate sterile solvent, the solution should be used promptly according to laboratory protocols.

Intended Use and Regulatory Status

Research Use Only

SEMAX is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating peptide interactions with cellular systems

• Neuroscience research — studying nootropic and neuroprotective mechanisms

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into peptide structure and function

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

SEMAX should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming and denaturation

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for SEMAX:
For researchers needing a 5mg/mL working solution, add 2mL of sterile solvent to the 10mg vial. For a 10mg/mL solution, add 1mL of solvent. For a 2mg/mL solution, add 5mL of solvent. For a 1mg/mL solution, add 10mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions. The choice of solvent may affect peptide stability and should be validated for each experimental application.

Disposal Considerations

Dispose of SEMAX and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

SEMAX is used in various laboratory research contexts, primarily focused on neuroscience and neuropharmacology. Typical applications include :

Neurotrophin and BDNF Research:
SEMAX has been shown to stimulate the synthesis of brain-derived neurotrophic factor (BDNF), a potent modulator of synaptic plasticity. Studies have demonstrated that SEMAX application results in a rapid increase in BDNF levels in specific brain regions. This BDNF-modulating property makes SEMAX a valuable research tool for investigators studying neurotrophic signaling pathways .

Neurotransmitter System Studies:
Research has revealed that SEMAX activates dopaminergic and serotoninergic brain systems in controlled laboratory models. Specifically, studies have shown:

• Significant increases in serotonin metabolite levels following SEMAX administration

• Positive modulatory effects on the serotonergic system

• Enhanced dopamine release when combined with other compounds
  These neurotransmitter-modulating properties are of significant interest to researchers studying monoaminergic systems .

Neuroprotective Mechanism Investigations:
SEMAX has been the subject of research into its neuroprotective properties. Studies using experimental models have shown that SEMAX:

• Enhances transcription of neurotrophins and their receptors

• Affects expression of genes associated with immune response

• Suppresses activation of genes involved in inflammatory processes

• Prevents decreases in expression of genes associated with neurotransmission

Amyloid Research:
SEMAX has been investigated for its ability to interact with amyloid structures. Studies have shown that SEMAX can inhibit fiber formation in both the presence and absence of metal ions. The peptide demonstrates anti-aggregating properties, making it a potential research tool for investigators studying protein aggregation mechanisms .

Cholinergic System Studies:
In vitro research has demonstrated that SEMAX:

• Increases survival of cholinergic basal forebrain neurons

• Stimulates activity of choline acetyltransferase in dissociated basal forebrain tissue cultures

• Does not affect GABA-ergic neurons or glial cell proliferation

Specific Binding Site Research:
Studies have identified specific binding sites for SEMAX in brain tissue. The binding has been characterized as time-dependent, specific, and reversible, requiring calcium ions. This binding site characterization provides a foundation for understanding the molecular mechanisms underlying SEMAX’s observed effects .

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to ACTH analog peptides.

What Makes This Product Different

Unique ACTH(4-10) Analog Structure

Unlike many research peptides, SEMAX is derived from the ACTH(4-10) fragment with a stabilizing Pro-Gly-Pro modification at the C-terminus. This unique structure provides greater metabolic stability compared to the native sequence, making it a valuable research tool for investigators studying ACTH-related pathways .

Rigorous Quality Control

Unlike research chemicals sold without verification, SEMAX comes with documented third-party testing for every batch. This commitment to quality assurance gives researchers confidence in the consistency and reliability of their experimental results .

Complete Documentation

Each product listing includes full chemical specifications, including molecular formula, molecular mass, CAS number, and amino acid sequence. This transparency allows researchers to verify the exact compound they are working with .

Appropriate Packaging for Research Use

The sterile, sealed vial packaging protects the lyophilized powder from environmental contaminants. The 10mg concentration is particularly useful for experiments requiring precise, smaller-scale handling.

Batch Traceability

Each package of SEMAX includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of SEMAX?

Each batch is tested to verify purity exceeding 98%. Individual certificates of analysis are available upon request .

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Due to the presence of methionine in the sequence, minimizing air exposure is particularly important. Avoid repeated freeze-thaw cycles once reconstituted.

What solvent should be used for reconstitution?

Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate-buffered saline (PBS). The choice of solvent may affect peptide stability.

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22-micron filter may be appropriate.

What is the significance of the CAS number 80714-61-0?

The CAS number is a unique identifier for SEMAX, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications .

What is the amino acid sequence of SEMAX?

The sequence is Methionine-Glutamic Acid-Histidine-Phenylalanine-Proline-Glycine-Proline, abbreviated as MEHFPGP .

How does SEMAX differ from native ACTH(4-10)?

SEMAX is a stabilized analog of ACTH(4-10) with a Pro-Gly-Pro modification at the C-terminus. This modification provides greater metabolic stability compared to the native sequence .

Can SEMAX be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. Standard laboratory practice includes testing interactions in control experiments before full-scale implementation.

Final Notes for Laboratory Researchers

SEMAX represents a high-purity research tool for qualified professionals conducting legitimate scientific investigations into nootropic mechanisms, neuroprotection, BDNF signaling, neurotransmitter systems, and related fields. With documented third-party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 10mg vial size is optimized for smaller-scale experiments, preliminary studies, and protocols requiring precise handling. For larger-scale research, multiple vials can be used to achieve the desired quantity.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.