NAD+ 500MG Research Grade — High-Purity Nicotinamide Adenine Dinucleotide for Laboratory Use

Product Overview

NAD+ (nicotinamide adenine dinucleotide) is a research-grade coenzyme supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 500mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 500mg per vial, 10 vials per pack

• Form: Lyophilized (freeze-dried) powder

• Purity: >99% verified by third-party testing

• Molecular Formula: C₂₁H₂₇N₇O₁₄P₂

• Molecular Mass: 663.43

• CAS Number: 53-84-9

• Synonyms: DPN, Cozymase, Codehydrogenase I, Nadide

• Research Use Only: Not for human or animal consumption

• NAD+ 500MG

Understanding NAD+: The Research Context

NAD+ (nicotinamide adenine dinucleotide) is a fundamental coenzyme found in all living cells. It plays an essential role in cellular metabolism and energy production. As a central molecule in redox reactions, NAD+ exists in two forms: the oxidized form (NAD+) and the reduced form (NADH). The ratio between these two forms is critical for cellular function. NAD+ 500MG

Key Research Interests:

• Cellular energy metabolism and bioenergetics

• Redox biology and electron transfer

• Enzyme kinetics and coenzyme-dependent reactions

• Sirtuin and PARP enzyme studies

• In vitro cell culture investigations

• Aging and longevity research

Researchers utilize NAD+ in controlled laboratory settings to study fundamental cellular processes including energy metabolism, DNA repair mechanisms, and calcium signaling pathways. The coenzyme is of particular interest to investigators focused on metabolic disorders, neurodegenerative diseases, and the basic biology of aging. NAD+ 500MG

The Significance of NAD+ in Cellular Biology:
NAD+ is one of the most abundant and critical molecules in cellular metabolism. It serves two primary functions in cells:

• Electron carrier: NAD+ accepts electrons during metabolic reactions such as glycolysis, the Krebs cycle, and fatty acid oxidation, becoming NADH. This electron transfer is essential for ATP production. NAD+ 500MG

• Substrate for signaling enzymes: NAD+ 500MG is consumed by several classes of enzymes, including sirtuins, PARPs (poly-ADP-ribose polymerases), and CD38, which regulate diverse cellular processes. NAD+ 500MG

The cellular levels of NAD+ decline with age and under various experimental conditions, making NAD+ metabolism a active area of basic research. NAD+ 500MG

Third-Party Testing and Quality Assurance

Every batch of NAD+ undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third-party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release. NAD+ 500MG

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high-performance liquid chromatography (HPLC)

• Batch-to-batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity exceeding 99%, NAD+ meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients. NAD+ 500MG

Chemical and Physical Properties

Molecular Characteristics

NAD+ is a dinucleotide consisting of two nucleotides joined by their phosphate groups. One nucleotide contains an adenine base, while the other contains a nicotinamide base. NAD+ 500MG

Detailed Molecular Information:

• Molecular Formula: C₂₁H₂₇N₇O₁₄P₂

• Molecular Mass: 663.43 daltons

• CAS Number: 53-84-9

• IUPAC Name: (2R,3S,4R,5R)-2-(3-carbamoylpyridin-1-ium-1-yl)-5-({[({[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl)oxy)methyl]oxolane-3,4-diol

• Melting Point: 140-142°C (decomposes)

• Solubility: Freely soluble in water

Structural Features:

• Contains a nicotinamide ring that can accept an electron and a proton to become NADH

• Contains an adenine nucleotide

• Linked by two phosphate groups (pyrophosphate linkage)

• Positively charged nicotinamide ring

Physical Form and Storage

The product is supplied as a lyophilized (freeze-dried) powder within a sterile, sealed vial. Lyophilization preserves the chemical integrity of NAD+ by removing water content while maintaining the compound’s structure. NAD+ 500MG

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long-term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze-thaw cycles once reconstituted

Stability Considerations:
NAD+ is hygroscopic (absorbs moisture from the air) and can degrade when exposed to humidity, heat, or light. Proper storage is essential to maintain purity. Once reconstituted in aqueous solution, NAD+ 500MG is less stable than in lyophilized form and should be used promptly or stored frozen in aliquots. NAD+ 500MG

Intended Use and Regulatory Status

Research Use Only

NAD+ is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating cellular metabolism and signaling

• Enzyme assays — studying NAD+ 500MG -dependent enzymes

• Biochemical research — redox reaction studies

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into cellular metabolism

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

NAD+ should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for NAD+ 500MG:
For researchers needing a 100mg/mL working solution, add 5mL of sterile solvent to the 500mg vial. For a 50mg/mL solution, add 10mL of solvent. For a 25mg/mL solution, add 20mL of solvent. Always verify calculations before proceeding with experiments. NAD+ 500MG

Solvent Considerations:
NAD+ is freely soluble in water. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate-buffered saline (PBS). NAD+ solutions are typically stable at neutral to slightly alkaline pH but degrade more rapidly under acidic conditions. NAD+ 500MG

Disposal Considerations

Dispose of NAD+ and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines. NAD+ 500MG

Research Applications

NAD+ is used in various laboratory research contexts, spanning biochemistry, cell biology, and molecular biology. Typical applications include:

Redox Biology and Metabolism Research:
NAD+ is central to cellular energy metabolism. Researchers use NAD+ to study:

• Glycolysis and the conversion of glucose to pyruvate

• The Krebs cycle (citric acid cycle) and oxidative metabolism

• Fatty acid beta-oxidation

• Electron transport chain function

• Lactate dehydrogenase (LDH) assays

• Alcohol dehydrogenase studies

Enzyme Kinetics and Assays:
NAD+ is a substrate or cofactor for numerous enzymes. Common research applications include:

• Sirtuin (SIRT1-7) enzyme activity assays

• PARP (poly-ADP-ribose polymerase) activity studies

• CD38 and CD157 enzyme characterization

• Glutamate dehydrogenase kinetics

• Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) studies

• Malate dehydrogenase assays

Cell Culture Studies:
NAD+ is used in controlled cell culture systems to investigate:

• Cellular responses to NAD+ modulation

• Metabolic flux and energy homeostasis

• Cell viability and proliferation

• Oxidative stress responses

• Mitochondrial function

Aging and Longevity Research:
The decline in NAD+ levels with age is a active area of basic research. NAD+ is used to study:

• Age-related changes in cellular metabolism

• Sirtuin-mediated pathways in aging models

• DNA repair mechanisms and genomic stability

• Cellular senescence markers and pathways

Neurobiology Research:
NAD+ plays important roles in neuronal function and survival. Researchers use NAD+ to study:

• Neuronal energy metabolism

• Axonal degeneration mechanisms

• Neuroprotective signaling pathways

• Calcium homeostasis in neurons

Analytical Chemistry:
NAD+ is used as a standard and reagent in analytical applications:

• HPLC method development

• Mass spectrometry calibration

• Enzymatic assay validation

• Metabolomics research

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to NAD+ biochemistry.

What Makes This Product Different

High Concentration 500MG Vials

Unlike standard research peptides supplied in 10mg vials, NAD+ is offered in 500mg vials. This higher quantity reflects the different research applications of NAD+, which is often used in larger quantities for enzyme assays, cell culture studies, and biochemical experiments.

Essential Cellular Coenzyme

NAD+ is not merely a research peptide but a fundamental coenzyme present in all living cells. Its central role in metabolism and cellular signaling makes it a critical reagent for a wide range of biological research applications.

Rigorous Quality Control

Unlike research chemicals sold without verification, NAD+ comes with documented third-party testing for every batch. This commitment to quality assurance gives researchers confidence in the consistency and reliability of their experimental results.

Complete Documentation

Each product listing includes full chemical specifications, including molecular formula, molecular mass, CAS number, and synonyms. This transparency allows researchers to verify the exact compound they are working with.

Appropriate Packaging for Research Use

The sterile, sealed vial packaging protects the lyophilized powder from environmental contaminants. The 500mg concentration is specifically designed for researchers who require larger quantities for enzyme assays, cell culture supplementation, or biochemical preparations.

Batch Traceability

Each package of NAD+ includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of NAD+?

Each batch is tested to verify purity exceeding 99%. Individual certificates of analysis are available upon request.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. NAD+ is hygroscopic, so keeping the vial tightly sealed and desiccated is particularly important.

What solvent should be used for reconstitution?

NAD+ is freely soluble in water. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as PBS at neutral pH.

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22-micron filter may be appropriate.

What is the difference between NAD+ and NADH?

NAD+ is the oxidized form of nicotinamide adenine dinucleotide, while NADH is the reduced form. The two forms differ by the addition of a hydride ion (H⁻) to the nicotinamide ring. Researchers use both forms depending on whether they need to study oxidation or reduction reactions.

What is the significance of the CAS number 53-84-9?

The CAS number is a unique identifier for NAD+, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing NAD+ in publications.

Why is NAD+ provided in 500mg vials rather than 10mg vials?

NAD+ is typically used in larger quantities than many research peptides due to its role as a coenzyme in enzyme assays and cell culture applications. The 500mg vial size reflects these different usage patterns.

How stable is NAD+ in solution?

NAD+ is less stable in aqueous solution than in lyophilized form. For optimal stability, reconstituted solutions should be used promptly or aliquoted and stored frozen at -80°C. Avoid repeated freeze-thaw cycles.

Can NAD+ be used in combination with other research reagents?

Researchers should determine compatibility based on their specific experimental design. Standard laboratory practice includes testing interactions in control experiments before full-scale implementation.

Final Notes for Laboratory Researchers

NAD+ represents a high-purity research tool for qualified professionals conducting legitimate scientific investigations into cellular metabolism, redox biology, enzyme kinetics, and related fields. With documented third-party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 500mg vial size is specifically designed for researchers requiring larger quantities for enzyme assays, cell culture supplementation, biochemical preparations, and high-throughput experiments. For smaller-scale research, alternative package sizes may be available.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.