GHK-Cu 100MG Research Peptide — High-Purity Copper-Binding Tripeptide for Laboratory Use

Product Overview

GHK-Cu is a research-grade naturally occurring copper-binding tripeptide. It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 100mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 100mg per vial, 10 vials per pack

• Form: Lyophilized (freeze-dried) powder

• Purity: >99% verified by third-party testing

• Molecular Formula: C₁₄H₂₃CuN₆O₄

• Molecular Mass: 401.91

• CAS Number: 49557-75-7

• Sequence: Glycine-Histidine-Lysine (Gly-His-Lys)

• Synonyms: Copper-GHK, Copper Tripeptide-1

• Research Use Only: Not for human or animal consumption

Understanding GHK-Cu 100MG: The Research Context

GHK-Cu is a naturally occurring copper-binding tripeptide consisting of three amino acids: glycine, histidine, and lysine. It was first isolated from human plasma in 1973 and has since become one of the most extensively studied peptides in regenerative research.

Key Research Interests:

• Gene expression modulation and regulation

• Collagen and elastin synthesis stimulation

• Extracellular matrix (ECM) remodeling

• Wound healing and tissue repair mechanisms

• Antioxidant defense pathway activation

• In vitro cell culture investigations

Researchers utilize GHK-Cu in controlled laboratory settings to study its role in gene regulation, tissue remodeling, and cellular signaling. The peptide is of particular interest to investigators focused on regenerative biology, extracellular matrix dynamics, and the fundamental mechanisms of tissue repair.

The Significance of GHK-Cu 100MG in Human Biology:
GHK-Cu is naturally present in human plasma at approximately 200 ng/mL (about 0.5 µM) in young adults. This level declines significantly with age, falling to around 80 ng/mL by age 60 and to approximately 10 ng/mL by age 80. This age-related decline parallels reductions in healing capacity and tissue quality, making GHK-Cu 100MG a molecule of significant research interest in aging biology and regenerative medicine.

The Role of Copper:
The copper ion (Cu²⁺) is not merely a passive passenger in this peptide. It is essential for GHK-Cu’s biological activity. The copper ion acts as a critical cofactor, enabling the peptide to interact with cellular signaling pathways, bind to DNA sequences, and regulate gene expression. Without copper, the tripeptide GHK has significantly different properties.

GHK-Cu 100MG vs. 50MG: Choosing the Right Quantity for Your Research

Researchers often ask which concentration best suits their laboratory protocols. The primary difference between GHK-Cu 100MG and 50MG is the mass of research compound per vial.

Choosing the Right Vial Size:

• GHK-Cu 100MG — Ideal for large-scale experiments, high-throughput screening, multiple replicated assays, extended studies requiring consistent batch material, and protocols requiring higher peptide concentrations

• GHK-Cu 50MG — Better suited for moderate-scale studies, preliminary investigations, and protocols requiring precise handling of smaller quantities

Both products share identical chemical properties, purity standards, and quality assurance protocols. The choice between them depends entirely on the specific requirements of your research design and the scale of your experiments.

Third-Party Testing and Quality Assurance

Every batch of GHK-Cu undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third-party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high-performance liquid chromatography (HPLC)

• Copper content verification to confirm proper chelation

• Batch-to-batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity exceeding 99%, GHK-Cu meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients.

Chemical and Physical Properties

Molecular Characteristics

GHK-Cu is a copper-binding tripeptide with a distinctive structure. The copper ion is chelated by the peptide, primarily through the histidine residue.

Detailed Molecular Information:

• Molecular Formula: C₁₄H₂₃CuN₆O₄

• Molecular Mass: 401.91 daltons

• CAS Number: 49557-75-7

• Sequence: Gly-His-Lys (Glycine-Histidine-Lysine)

• Copper Content: Approximately 15.8% by mass

Amino Acid Properties:

Structural Features:

• Linear tripeptide (3 amino acids)

• Copper ion (Cu²⁺) chelated to the peptide

• Histidine residue provides primary coordination for copper

• Positively charged lysine residue

• Pale blue to blue-green color due to copper content

Coordination Chemistry:
The copper ion is coordinated by the imidazole nitrogen of histidine, the amino terminus nitrogen, and two backbone amide nitrogens. This chelation creates a stable complex that protects the copper ion and enables specific biological interactions.

Physical Form and Storage

The product is supplied as a lyophilized (freeze-dried) powder within a sterile, sealed vial. The powder typically appears as a pale blue to blue-green substance due to the copper content.

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long-term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze-thaw cycles once reconstituted

Stability Considerations:
GHK-Cu is stable when stored properly in lyophilized form. The copper-peptide bond is stable at neutral pH. Under acidic conditions (below pH 3.5), the copper may dissociate from the peptide. For most laboratory applications using neutral buffers (pH 7.0-7.4), the complex remains intact.

Once reconstituted with an appropriate sterile solvent, the solution should be used promptly according to laboratory protocols.

Intended Use and Regulatory Status

Research Use Only

GHK-Cu is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating gene expression and cellular signaling

• Gene expression research — studying modulation of approximately 31% of human genes

• Extracellular matrix studies — collagen and elastin synthesis research

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into peptide structure and function

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities.

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

GHK-Cu should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for GHK-Cu 100MG:
For researchers needing a 100mg/mL working solution, add 1mL of sterile solvent to the 100mg vial. For a 50mg/mL solution, add 2mL of solvent. For a 25mg/mL solution, add 4mL of solvent. For a 10mg/mL solution, add 10mL of solvent. Always verify calculations before proceeding with experiments. GHK-Cu 100MG

Solvent Considerations:
GHK-Cu is water-soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as phosphate-buffered saline (PBS) at neutral pH (7.0-7.4). Avoid acidic solvents (below pH 3.5) which may cause copper dissociation. GHK-Cu 100MG

Disposal Considerations

Dispose of GHK-Cu and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

GHK-Cu is used in various laboratory research contexts, spanning genomics, cell biology, and tissue engineering. Typical applications include:

Gene Expression Studies:
GHK-Cu is a well-documented modulator of gene expression. Research has demonstrated that GHK-Cu affects approximately 31% of human genes with ≥50% expression changes. This broad regulatory role makes GHK-Cu valuable for studying:

• Gene expression patterns in response to peptide treatment

• Transcriptional regulation mechanisms

• Upregulation of beneficial genes (collagen, elastin, antioxidants)

• Downregulation of inflammatory genes

Collagen and Elastin Synthesis Research:
GHK-Cu is known to stimulate the production of extracellular matrix components. Researchers use GHK-Cu to study:

• Collagen type I synthesis and organization

• Elastin production and assembly

• Extracellular matrix remodeling dynamics

• Fibroblast activation and function

Wound Healing and Tissue Repair Studies:
GHK-Cu has been extensively studied in tissue repair models. Research applications include:

• Wound healing kinetics and mechanisms

• Angiogenesis and blood vessel formation

• Granulation tissue formation

• Re-epithelialization processes

Antioxidant and Anti-Inflammatory Research:
GHK-Cu modulates oxidative stress and inflammatory pathways. Researchers use GHK-Cu 100MG to investigate:

• Antioxidant enzyme expression (SOD, catalase, glutathione peroxidase)

• Reactive oxygen species (ROS) regulation

• Pro-inflammatory cytokine modulation

• NF-κB pathway interactions

Fibroblast and Keratinocyte Cell Culture Studies:
GHK-Cu is used in controlled cell culture systems to study:

• Fibroblast proliferation and migration

• Keratinocyte differentiation and function

• Cellular responses to copper-peptide signaling

• Extracellular matrix production

Aging Biology Research:
Given the age-related decline in plasma GHK-Cu 100MG levels, researchers use GHK-Cu to study:

• Age-related changes in tissue repair capacity

• Cellular senescence markers

• Extracellular matrix degradation in aging models

• Regenerative decline mechanisms

Large-Scale and High-Throughput Applications:
The 100MG vial size enables:

• High-throughput screening assays requiring large peptide quantities

• Extended time-course studies with consistent batch material

• Multi-well plate experiments (96-well, 384-well formats)

• Repeated experiments without batch-to-batch variation

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to copper-binding peptides.

What Makes This Product Different

Naturally Occurring Human Peptide

Unlike many synthetic research peptides, GHK-Cu is a naturally occurring peptide found in human plasma. This natural origin provides a relevant biological context for research into its mechanisms and functions. GHK-Cu 100MG

Broad Gene Expression Modulation

GHK-Cu affects approximately 31% of human genes with ≥50% expression changes, making it one of the most broadly active regulatory peptides identified. This broad activity distinguishes GHK-Cu from peptides with narrow, receptor-specific actions. GHK-Cu 100MG

Copper-Dependent Activity

The copper ion is essential for GHK-Cu’s biological activity. Unlike peptides that function solely through their amino acid sequence, GHK-Cu requires proper copper chelation for gene regulatory and signaling functions.

Age-Related Decline Significance

Plasma GHK-Cu levels decline significantly with age, from approximately 200 ng/mL in young adults to about 10 ng/mL by age 80. This age-related decline makes GHK-Cu a molecule of significant interest in aging biology research.

High-Capacity 100MG Vials for Large-Scale Research

The 100MG vial size is specifically designed for researchers conducting large-scale experiments, high-throughput screening, extended studies, or protocols requiring substantial peptide quantities. This higher capacity reduces the number of vials needed for extensive research programs. GHK-Cu 100MG

Rigorous Quality Control

Unlike research chemicals sold without verification, GHK-Cu comes with documented third-party testing for every batch. This commitment to quality assurance gives researchers confidence in the consistency and reliability of their experimental results.

Distinctive Physical Appearance

The pale blue to blue-green color of GHK-Cu is a distinctive visual indicator of proper copper chelation, providing researchers with immediate visual confirmation of product integrity. GHK-Cu 100MG

Batch Traceability

Each package of GHK-Cu includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation. GHK-Cu 100MG

Frequently Asked Questions for Researchers

What is the purity level of GHK-Cu 100MG?

Each batch is tested to verify purity exceeding 99%. Individual certificates of analysis are available upon request.

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Avoid repeated freeze-thaw cycles once reconstituted.

What solvent should be used for reconstitution?

GHK-Cu is water-soluble. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, or buffered solutions such as PBS at neutral pH (7.0-7.4). Avoid acidic solvents below pH 3.5.

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22-micron filter may be appropriate.

What is the significance of the CAS number 49557-75-7?

The CAS number is a unique identifier for GHK-Cu (Copper Tripeptide-1), allowing researchers to verify they are working with the correct chemical compound when ordering or referencing the peptide in publications.

What is the amino acid sequence of GHK-Cu 100MG?

The sequence is Glycine-Histidine-Lysine, abbreviated as GHK or Gly-His-Lys.

Why does GHK-Cu appear blue?

The pale blue to blue-green color is characteristic of copper complexes. The copper ion (Cu²⁺) in GHK-Cu absorbs light in the red-orange region, resulting in the complementary blue color visible to researchers.

What is the difference between GHK-Cu 100MG and 50MG?

The only difference is the quantity per vial. GHK-Cu 100MG contains 100mg per vial, while GHK-Cu 50MG contains 50mg per vial. Both share identical chemical properties, purity standards (>99%), and quality assurance protocols.

Which vial size should I choose for my research?

Choose 100MG for large-scale experiments, high-throughput screening, extended studies, multiple replicated assays, or protocols requiring higher peptide concentrations. Choose 50MG for moderate-scale studies, preliminary investigations, or when precise handling of smaller quantities is preferred.

Can GHK-Cu 100MG be used in combination with other research peptides?

Researchers should determine compatibility based on their specific experimental design. GHK-Cu is frequently studied in combination with BPC-157 and TB-500 in multi-peptide formulations such as GLOW and KLOW. Standard laboratory practice includes testing interactions in control experiments.

Final Notes for Laboratory Researchers

GHK-Cu 100MG represents a high-purity research tool for qualified professionals conducting legitimate scientific investigations into gene expression modulation, extracellular matrix remodeling, tissue repair mechanisms, aging biology, and related regenerative fields. With documented third-party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 100MG vial size is specifically optimized for large-scale research applications requiring substantial peptide quantities, including high-throughput screening, extended time-course studies, multi-well plate experiments, and protocols demanding consistent batch material across numerous assays.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.