5-Amino-1MQ Research Compound — High-Purity NNMT Inhibitor for Laboratory Use

Product Overview

5-Amino-1MQ (5-Amino-1-methylquinolinium) is a research-grade small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT). It is supplied as a sterile lyophilized powder in sealed vials. Each package contains ten vials, with each vial holding 10mg of the research compound. This product is manufactured exclusively for scientific investigation and in vitro laboratory experimentation.

Key Specifications at a Glance:

• Quantity: 10mg per vial, 10 vials per pack

• Form: Lyophilized (freeze-dried) powder

• Purity: >98% verified by third-party testing

• CAS Number: 42464-96-0

• Chemical Name: 5-Amino-1-methylquinolin-1-ium iodide

• Molecular Formula: C₁₀H₁₁N₂·I (iodide salt)

• Synonyms: 5A1MQ, NNMTi, 5-Amino-1-methylquinolinium

• Research Use Only: Not for human or animal consumption

Understanding 5-Amino-1MQ: The Research Context

5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme involved in NAD⁺ metabolism and cellular energy regulation. Unlike traditional peptides that function as signaling molecules or receptor agonists, 5-Amino-1MQ works by inhibiting a specific metabolic enzyme, making it a valuable tool for metabolic research .

Key Research Interests:

• NNMT enzyme inhibition and metabolic pathway studies

• NAD⁺ metabolism and cellular energy regulation

• Adipose tissue metabolism and lipid storage

• Muscle stem cell differentiation and regeneration

• In vitro cell culture investigations

Researchers utilize 5-Amino-1MQ in controlled laboratory settings to study the role of NNMT in metabolism, cellular differentiation, and aging-related processes. The compound is of particular interest to investigators focused on metabolic disorders, regenerative medicine, and the fundamental mechanisms of cellular energy homeostasis .

The Significance of NNMT Inhibition:
Nicotinamide N-methyltransferase (NNMT) is an enzyme that methylates nicotinamide, a derivative of vitamin B3, using S-adenosylmethionine (SAM) as a methyl donor. This reaction consumes SAM and produces S-adenosylhomocysteine (SAH), impacting methylation potential and NAD⁺ metabolism .

By inhibiting NNMT, 5-Amino-1MQ may:

• Increase intracellular nicotinamide and NAD⁺ levels

• Support enhanced cellular energy production

• Promote metabolic shifts in adipose tissue

• Influence muscle stem cell differentiation

Third-Party Testing and Quality Assurance

Every batch of 5-Amino-1MQ undergoes rigorous independent analytical testing to verify identity, purity, and consistency. This third-party testing ensures that researchers receive a product that meets strict quality specifications before it is approved for release.

The testing process includes:

• Identity verification through mass spectrometry

• Purity analysis using high-performance liquid chromatography (HPLC)

• Batch-to-batch consistency checks

• Residual solvent analysis to confirm complete lyophilization

With a verified purity exceeding 98%, 5-Amino-1MQ meets the high standards required for serious laboratory research applications. Each vial contains only the lyophilized research compound with no unnecessary additives, fillers, or excipients .

Chemical and Physical Properties

Molecular Characteristics

5-Amino-1MQ is a small quinolinium-based molecule, not a peptide. It is typically supplied as an iodide salt for stability .

Detailed Molecular Information:

• CAS Number: 42464-96-0

• Chemical Name: 5-Amino-1-methylquinolin-1-ium iodide

• Molecular Formula: C₁₀H₁₁N₂·I

• Purity: >98% (HPLC)

Mechanism of Action:
5-Amino-1MQ is a substrate site-targeting, selective NNMT inhibitor with an IC₅₀ of 1.2 μM (tested with 50 μM SAM and 100 μM nicotinamide). It binds selectively to substrate-binding site residues of NNMT without affecting related methyltransferases or enzymes in the NAD⁺ salvage pathway .

Physical Form and Storage

The product is supplied as a lyophilized (freeze-dried) powder within a sterile, sealed vial. The powder typically appears as an off-white to pale substance .

Recommended Storage Conditions:

• Store lyophilized powder at -20°C (-4°F) or below for long-term preservation

• Protect from light exposure

• Keep desiccated to prevent moisture absorption

• Allow vial to reach room temperature before opening to avoid condensation

• Avoid repeated freeze-thaw cycles once reconstituted

Reconstituted Solution Storage:
Reconstituted solutions should be stored at 4°C and used within a few days, or aliquoted and frozen for extended storage. Avoid repeated freeze-thaw cycles .

Intended Use and Regulatory Status

Research Use Only

5-Amino-1MQ is classified as a research chemical and is intended exclusively for laboratory and in vitro experimentation. This designation means the product is sold strictly for:

• In vitro testing — experiments conducted in controlled laboratory environments

• Cell culture studies — investigating NNMT inhibition in metabolic pathways

• Metabolic research — studying NAD⁺ metabolism and energy regulation

• Muscle regeneration studies — investigating myoblast differentiation

• Analytical research — chemical analysis and characterization studies

• Scientific investigation — basic research into enzyme inhibition

Important Regulatory Notice

This product is not a drug, food, cosmetic, or medical device. Bodily introduction of any kind into humans or animals is strictly forbidden by law. The product should only be handled by licensed, qualified professionals working in appropriate laboratory facilities .

The supplier is a chemical supplier, not a compounding pharmacy or outsourcing facility under Sections 503A or 503B of the Federal Food, Drug, and Cosmetic Act. All product information provided is for educational purposes only and does not constitute medical advice or clinical recommendations.

Handling and Safety Guidelines

Professional Handling Required

5-Amino-1MQ should only be handled by trained laboratory personnel wearing appropriate personal protective equipment (PPE), including:

• Laboratory coat

• Nitrile gloves

• Safety goggles or face shield

• Appropriate respiratory protection if aerosolization is possible

Reconstitution Protocol

For laboratory use, the lyophilized powder should be reconstituted with a sterile, compatible solvent. Standard practice includes:

1. Allow the sealed vial to reach room temperature — approximately 15-30 minutes

2. Clean the rubber stopper with an alcohol swab

3. Add the calculated volume of solvent to achieve desired concentration

4. Gently swirl or roll the vial between palms — do not shake vigorously to avoid foaming

5. Allow complete dissolution before use — this typically takes 2-5 minutes

Reconstitution Example for 5-Amino-1MQ 10MG:
For researchers needing a 10mg/mL working solution, add 1mL of sterile solvent to the 10mg vial. For a 5mg/mL solution, add 2mL of solvent. For a 2mg/mL solution, add 5mL of solvent. For a 1mg/mL solution, add 10mL of solvent. Always verify calculations before proceeding with experiments.

Solvent Considerations:
5-Amino-1MQ is slightly soluble in water and DMSO . Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water for injection, bacteriostatic water, DMSO, or buffered solutions such as phosphate-buffered saline (PBS).

Disposal Considerations

Dispose of 5-Amino-1MQ and any contaminated materials in accordance with federal, state, and local regulations for chemical waste disposal. Do not dispose of in regular trash or down drains unless permitted by local guidelines.

Research Applications

5-Amino-1MQ is used in various laboratory research contexts, primarily focused on metabolic regulation and cellular differentiation. Typical applications include:

NNMT Enzyme Inhibition Studies:
The primary research interest in 5-Amino-1MQ revolves around its selective inhibition of NNMT. Researchers use 5-Amino-1MQ to study:

• NNMT enzyme kinetics and inhibition mechanisms

• Substrate binding site interactions

• Selectivity profiles against related methyltransferases

• Concentration-response relationships (IC₅₀ = 1.2 μM)

NAD⁺ Metabolism Research:
By inhibiting NNMT, 5-Amino-1MQ impacts NAD⁺ metabolism. Research applications include:

• NAD⁺ salvage pathway studies

• Cellular energy metabolism investigations

• Nicotinamide and methylation balance research

Adipocyte and Metabolic Studies:
5-Amino-1MQ has been studied in metabolic research models. In vitro studies have demonstrated:

• Reduction of 3T3-L1 lipogenesis with an EC₅₀ of 30 μM

• Decreased adipocyte 1-methylnicotinamide levels with an EC₅₀ of 2.3 μM

• No effect on related metabolic enzymes

Muscle Regeneration Research:
5-Amino-1MQ has been investigated for its effects on muscle stem cells. Research demonstrates:

• Concentration-dependent increase in C2C12 myoblast differentiation (10-30 μM; 96 hours)

• 45% increase in MHC-positive myotube nuclei at 30 μM compared to untreated cells

• Enhanced muscle stem cell fusion and regeneration

Cell Culture Investigations:
Researchers use 5-Amino-1MQ in controlled cell culture systems to study:

• Myoblast differentiation pathways

• NNMT expression and regulation

• Cellular responses to metabolic modulation

• Gene expression changes following NNMT inhibition

In Vivo Research Models:
Preclinical research has utilized 5-Amino-1MQ in animal models with the following observations:

• Therapeutic efficacy in murine models of diet-induced obesity (20 mg/kg/day, subcutaneous)

• Improved muscle regeneration post-injury (5 or 10 mg/kg twice daily, subcutaneous)

• 60% and 75% higher incidence of proliferating/active muscle stem cells at 5 mg/kg and 10 mg/kg

• No systemic toxicity observed even after daily dosing for 1 week

Researchers should design their experimental protocols based on peer-reviewed literature and established laboratory practices specific to NNMT inhibitors.

What Makes This Product Different

Small-Molecule NNMT Inhibitor

Unlike many research peptides that function as receptor agonists or signaling molecules, 5-Amino-1MQ is a small-molecule enzyme inhibitor targeting NNMT. This distinct mechanism of action makes it valuable for metabolic research applications.

Selective Inhibition Profile

5-Amino-1MQ demonstrates high selectivity for NNMT with minimal off-target effects. It does not affect related methyltransferases or enzymes in the NAD⁺ salvage pathway, allowing researchers to study NNMT-specific effects .

Well-Characterized In Vitro Activity

Extensive in vitro characterization provides researchers with validated experimental parameters:

• IC₅₀: 1.2 μM for NNMT inhibition

• EC₅₀ (lipogenesis reduction): 30 μM

• EC₅₀ (1-methylnicotinamide reduction): 2.3 μM

Favorable Safety Profile in Research Models

Preclinical studies have demonstrated that 5-Amino-1MQ is well-tolerated in animal models with no observed systemic toxicity at doses up to 60 mg/kg/day. Research subjects maintained normal levels of glucose, cholesterol, plasma proteins, and electrolytes .

Rigorous Quality Control

Unlike research chemicals sold without verification, 5-Amino-1MQ comes with documented third-party testing for every batch. This commitment to quality assurance gives researchers confidence in the consistency and reliability of their experimental results .

Complete Documentation

Each product listing includes full chemical specifications, including CAS number, molecular formula, and purity certification. This transparency allows researchers to verify the exact compound they are working with.

Appropriate Packaging for Research Use

The sterile, sealed vial packaging protects the lyophilized powder from environmental contaminants. The 10mg concentration is particularly useful for experiments requiring precise, smaller-scale handling.

Batch Traceability

Each package of 5-Amino-1MQ includes batch identification numbers that allow researchers to trace their material back to original manufacturing and testing records. This traceability is essential for reproducible research and quality documentation.

Frequently Asked Questions for Researchers

What is the purity level of 5-Amino-1MQ?

Each batch is tested to verify purity exceeding 98% by HPLC. Individual certificates of analysis are available upon request .

Is 5-Amino-1MQ a peptide?

No, 5-Amino-1MQ is a small-molecule quinolinium compound, not a peptide. It is a nicotinamide N-methyltransferase (NNMT) inhibitor .

How should the lyophilized powder be stored?

Store at -20°C or below, protected from light and moisture. Avoid repeated freeze-thaw cycles once reconstituted.

What solvent should be used for reconstitution?

5-Amino-1MQ is slightly soluble in water and DMSO. Researchers should determine the appropriate solvent based on their specific experimental protocol. Common options include sterile water, DMSO, or buffered solutions such as PBS .

Is this product sterile?

The lyophilized powder is manufactured under aseptic conditions, but researchers should follow standard laboratory practices for handling research chemicals. For cell culture applications, additional filtration using a 0.22-micron filter may be appropriate.

What is the significance of the CAS number 42464-96-0?

The CAS number is a unique identifier for 5-Amino-1MQ, allowing researchers to verify they are working with the correct chemical compound when ordering or referencing it in publications .

What is the mechanism of action of 5-Amino-1MQ?

5-Amino-1MQ is a selective inhibitor of nicotinamide N-methyltransferase (NNMT), binding to the substrate-binding site of the enzyme with an IC₅₀ of 1.2 μM .

What research applications is 5-Amino-1MQ used for?

5-Amino-1MQ is studied in metabolic research, NAD⁺ metabolism, adipocyte biology, muscle regeneration, and myoblast differentiation in laboratory cell culture and preclinical models .

Can 5-Amino-1MQ be used in combination with other research compounds?

Researchers should determine compatibility based on their specific experimental design. Standard laboratory practice includes testing interactions in control experiments before full-scale implementation.

Final Notes for Laboratory Researchers

5-Amino-1MQ represents a high-purity research tool for qualified professionals conducting legitimate scientific investigations into NNMT enzyme inhibition, NAD⁺ metabolism, adipose tissue biology, muscle regeneration, and related metabolic fields. With documented third-party testing, complete chemical specifications, and appropriate packaging for laboratory use, this product meets the standards expected by serious researchers.

The 10mg vial size is optimized for smaller-scale experiments, preliminary studies, and protocols requiring precise handling. For larger-scale research, multiple vials can be used to achieve the desired quantity.

For research use only. Not for human or animal consumption. Handle only in appropriate laboratory facilities with proper safety equipment and training.